Table 2.

The nlxA gene is involved in stress resistance against H₂O₂, polymyxin B, sodium dodecylsulphate (SDS), phenol, CuSO₄ and ZnSO₄.

Strains		Bacterial concentration before challenge (108 CFU/mL)*, †	Survival percentage (%) after challenge*
Name	Genotype		H2O2	Polymyxin B	SDS	Phenol	CuSO4	ZnSO4
306/pUFR053	WT	9.33 ± 0.58a	5.76 ± 1.19a	14.81 ± 1.28a	0.57 ± 0.03a	93.51 ± 5.78a	1.02 ± 0.42a	1.42 ± 0.32a
406G8/pUFR053	nlxA−	10.33 ± 0.85a	0.85 ± 0.04b	3.84 ± 1.61b	0.07 ± 0.03b	41.76 ± 12.61b	0.18 ± 0.10b	0.37 ± 0.08b
406G8/p53‐nlxA	nlxA+	10.67 ± 1.53a	10.48 ± 4.62a	14.21 ± 2.42a	0.42 ± 0.13a	95.83 ± 7.22a	1.00 ± 0.57a	1.47 ± 0.19a

All the experiments were repeated three times independently with four replications each time. Similar results were observed and only one representative result is presented.

^* For significant difference analysis, the survival percentages of four replications of each strain were calculated independently and then subjected to Student's t‐test. Data with different letters (a, b) denote significant difference (P < 0.01) between strains in the same treatment. Means and standard errors from one representative result are shown.

^† All strains were cultured for 20 h in nutrient broth (NB). Initial cell concentrations [colony‐forming units (CFU)/mL] were measured by diluting the bacterial cultures appropriately and plating on nutrient agar (NA) plates before challenge with different stresses. For each strain tested, similar cell concentrations were observed in different stress assays. Only one set of the representative data is shown.