Table 3.
The expression of nlxA and other lipopolysaccharide (LPS) biosynthesis genes was induced on semi‐solid agar plates.
| Target gene† | Locus tag† | Annotated function | Primer sequence (5' to 3') | Fold change ± SE* | P value (Student's t‐test) |
|---|---|---|---|---|---|
| nlxA | NA | F: AGCCATGCAAACATCGTGTCGTTG | 2.611095 ± 0.705173 | 0.0304023 | |
| R: AAACGTAGGCATCGTCTAAGGCCA | |||||
| NA | XAC3588 | Integral membrane protein | F: TCGGAATTTCTACTGCAGACGCCA | 2.694485 ± 0.200239 | 0.0005489 |
| R: CGAATTCTGCAGCAAGCATGGACA | |||||
| NA | XAC3590 | Oxidoreductase | F: TCCTTCGGGCTTTCTGCAGTATCA | 3.367230 ± 0.324939 | 0.001889 |
| R: TGTTGCCGAATTCCTTCAACACGG | |||||
| wxacO | XAC3596 | Putative transmembrane protein | F: TCTTTATGCGCTCTTGGCCGTAGT | 5.199567 ± 0.647125 | 0.0128718 |
| R: ACATCGGTCATCTGGCGGACATAA | |||||
| rfbC | XAC3598 | Truncated O‐antigen biosynthesis protein | F: ATCCATCACCAGCACCTGTTCGTA | 2.772365 ± 0.842772 | 0.0052181 |
| R: GAATCCGCCAATGGCATCGAAGTT | |||||
| wzm | XAC3601 | ABC transporter permease | F: TTGAAATGGTCCCGATGCTGCTTG | 2.393728 ± 0.488077 | 0.0213009 |
| R: AAAGCAGAACGCTGCTCAGAATGC |
F, forward primer; R, reverse primer.
*
The wild‐type was cultured in nutrient broth (NB) for 20 h or on a semi‐solid plate for 24 h; the fold change was calculated using gene expression of the wild‐type after culture in NB for 20 h as calibrator according to the formula 2–ΔΔ CT (Livak and Schmittgen, 2001). SE, standard error.
†
Target genes and the corresponding ID are listed. NA denotes a gene name or ID that has not yet been assigned.