Table 4.
Bacterial strains, plasmids and primers for complementation.
| Strains or plasmids | Relevant characteristic* | Reference or source |
|---|---|---|
| Escherichia coli strain | ||
| EC100D pir+ | F‐ mcrAΔ(mrr‐hsdRMS‐mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara‐leu)7697 galU galKλ ‐ rpsL nupG pir+ DHFR | Epicentre |
| Xanthomonas citri ssp. citri strains | ||
| 306 | Wild‐type; Rifr | DPI |
| 406G8 | nlxA::EZ‐Tn5 derivative of strain 306; Rifr Kmr | Yan and Wang (2011b) |
| 415H9 | nlxA::EZ‐Tn5 derivative of strain 306; Rifr Kmr | Yan and Wang (2011b) |
| 330D3 | wzt::EZ‐Tn5 derivative of strain 306; Rifr Kmr | Yan and Wang (2011b) |
| Plasmids | ||
| pUFR053 | Shuttle vector, IncW Mob+ mob(P) lacZ+Par+ Gmr, | El Yacoubi et al. (2007) |
| p53‐nlxA | A 1362 bp EcoRI‐HindIII fragment containing wild‐type nlxA gene was ligated into pUFR053. Gmr, | This study |
| p53‐3597‐1 | A 1069 bp SacI‐HindIII fragment containing wild‐type XAC3597 gene was ligated into pUFR053. Gmr, | This study |
| p53‐3597‐2 | A 1339 bp SacI‐HindIII fragment containing wild‐type XAC3597 was ligated into pUFR053. Gmr, | This study |
| Primers | ||
| nlxAF1 | 5'‐TAGAATTCATCGTGAATCCGGAGATCCGTTC (EcoRI) | This study |
| nlxAR1 | 5'‐ATAAGCTTAACAACAAGGACTACAGCTGG (HindIII) | This study |
| 3597F1 | 5'‐TCGAGCTCGAATCAAGGCATATGCTCTCG (SacI) | This study |
| 3507F2 | 5'‐TTGAGCTCGATCTCGGGCCGTTCGTCCTG (SacI) | This study |
| 3597R1 | 5'‐TTCAAGCTTACGCGAGCCACTTACCAC (HindIII) | This study |
DPI, Division of Plant Industry of the Florida Department of Agriculture and Consumer Services, Gainesville, FL, USA.
*
Gmr, Kmr and Rifr indicate resistance to gentamycin, kanamycin and rifamycin, respectively; for primers, specific restriction sites of the primers are shown in italic and the names of the restriction enzymes are given.