Figure 4.

Comparison of Rad2b‐Pro and Rad2b‐Leu in suppression of local and systemic silencing of green fluorescence protein. (A) GFP fluorescence (5 days post‐infiltration) in leaves of N icotiana benthamiana  GFP transgenic line 16c with agroinfiltration of reporter plasmid p35S:GFP, together with control plasmid p35S:GUS, or a pBI121‐derived vector encoding 2b proteins, as indicated. (B) Northern blot analyses of GFP mRNA and GFP‐derived short‐interfering (si)RNA from the agroinfiltrated patches, and the patch treated with infiltration solution. Total RNA was extracted at 5 days post‐infiltration. (C) GFP fluorescence (14 days post‐infiltration) in the upper noninfiltrated leaves of N . benthamiana  GFP transgenic line 16c with agroinfiltration as in (A). Photographs were taken under UV light. (D) Northern blot analyses of GFP mRNA and siRNAs from the upper noninfiltrated leaves in (C). Total RNA was extracted at 14 days post‐infiltration. Total RNAs for detection of GFP mRNA and GFP‐derived siRNA were visualized by staining with ethidium bromide. The numbers above the detection of GFP siRNA represent the relative accumulation (RA) of GFP siRNA. The RA value of GFP siRNA from the patch expressing β‐glucuronidase (GUS) was set as unity.