Figure 5.

Self‐interaction of 2b proteins in vivo. N icotiana benthamiana  RDR 6i was co‐agroinfiltrated to transiently express together 2b proteins (Rad2b‐Pro and Rad2b‐Leu) fused to either the N (Rad2b‐Pro‐YFP^N and Rad2b‐Leu‐YFP^N) or C (Rad2b‐Pro‐YFP^C and Rad2b‐Leu‐YFP^C) terminus of yellow fluorescent protein (YFP). Also shown are controls in which the unfused YFP fragments (YFP^C and YFP^N) were co‐expressed, or expressed with one or other of the 2b‐YFP fusion proteins. Cells in infiltrated tissues were imaged at 5 days after agroinfiltration by confocal scanning laser microscopy for YFP fluorescence and under bright field microscopy. The bar represents 25 μm.