Table 1.
Primers used. The primers were designed with ePrimer (National Center for Biotechnology Information, NCBI)
| Organism | Targeted region | Use | Primer name | Sequence | Reference |
|---|---|---|---|---|---|
| Polymyxa betae | Internal transcribed spacer 1 | qPCR |
Pb1 Psp2rev |
5′‐GGAATTTGAACAAGTGACTTGG‐3′ 5′‐AGGGCTCTCGAAAGCGCAA‐3′ |
Legrève et al., 2003 |
| Beta vulgaris | Glutamine synthetase gene | qPCR |
GSBvFor GSBvRev |
5′‐AGGGTGATTGGAATGGTGCT‐3′ 5′‐ACTTCTCGATGGCAGCCTTT‐3′ |
This study |
| Beta vulgaris | NPR‐1 gene | RT‐PCR |
NPR1BvF NPR1BvR |
5′‐TCATGAAGCTTGTCGTCCTG‐3′ 5′‐ATACACCTTGCCAGCAATCC‐3′ |
This study |
| Beta vulgaris | PR‐8 gene (class III chitinase) | RT‐PCR |
Chit3BvF Chit3BvR |
5′‐GCTGAACTTAGCTGGGCACT‐3′ 5′‐CTGGACTGACCCCCAAGATA‐3′ |
This study |
qPCR, quantitative polymerase chain reaction; RT‐PCR, reverse transcription‐polymerase chain reaction.