Table 2.
Experiment | Zoospores inoculated | Difference in P. betae‐normalized C q | Mean sugar beet C q | ΔCycles | Reduction factor | Reduction rate |
---|---|---|---|---|---|---|
1 | 500 | 0.195 | 20.436 | 3.98 | 15.78 | 93.66 |
1 | 5000 | 0.151 | 20.32 | 3.07 | 8.39 | 88.08 |
2 | 500 | 0.092 | 21.6 | 1.99 | 3.97 | 74.82 |
2 | 5000 | 0.169 | 21.61 | 3.65 | 15.67 | 93.61 |
The reduction rate is reported as the difference between the P. betae‐normalized C q of treated plants and the P. betae‐normalized C q of the controls multiplied by the mean of the Beta vulgaris C q. It represents the standardized difference in C q (ΔCycles). At each amplification cycle, the DNA quantity is multiplied by two. The reduction factor can be obtained as 2ΔCycles. Finally, the reduction rate is obtained b: reduction rate = 1 − 1/reduction factor.