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. 2013 Sep 11;15(2):122–132. doi: 10.1111/mpp.12070

Figure 1.

figure

Comparison of chitinase activity from the chi54 mutant (C61Chi54M) and the mutant (C6IChi54TS) compared with the wild‐type (C61) strain of Chromobacterium sp. C61. Coomassie blue staining (A), Western blot (B) and chitinase activity staining (C) of proteins from C61 separated by sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Proteins (20 μg) were partially purified from culture filtrates of the wild‐type and mutant strains (C61, C61Chi54M and C61Chi54TS) grown in Luria–Bertani (LB) broth containing 0.2% colloidal chitin.