Table 2.

Translocation in planta is not dependent on HpaP. Translocation assay of type III effectors (T3Es) AvrA, GALA7, PopP1 and PopP2 in Nicotiana tabacum leaves using T3E‐CyaA′ fusion proteins in the wild‐type strain (WT), hpaP mutant and hrcV mutant (a type III secretion system‐defective mutant). Cyclic adenosine monophosphate (cAMP) levels (nmol cAMP/mg of total soluble proteins) were measured using the cAMP Biotrak competitive enzyme immunoassay in four independent biological replicates. Average ratios and standard errors of translocated protein in the wild‐type compared with the hpaP mutant were calculated for the four replicates

	WT	hrcV/WT	hpaP/WT
AvrA	100	0.04 ± 0.03	183 ± 149
GALA7	100	0.09 ± 0.07	126 ± 132
PopP1	100	0.03 ± 0.02	48 ± 28
PopP2	100	1 ± 1.5	104 ± 63