Skip to main content
. 2013 Apr 11;14(6):567–575. doi: 10.1111/mpp.12029

Figure 2.

figure

Control of ARGONAUTE 1 (AGO1) level is a universal phenomenon in virus infection. Systemically infected leaves of crucifer‐infecting Tobamovirus (crTMV)‐, Tobacco etch virus (TEV)‐, Turnip crinkle virus (TCV)‐ and Cucumber mosaic virus (CMV)‐inoculated Nicotiana benthamiana and CMV‐inoculated Arabidopsis thaliana plants were homogenized and used for RNA and protein extractions. The samples were used for the detection of AGO1 mRNA, AGO1 and miR168 expression. (A) Relative virus accumulation visualized by ethidium bromide‐stained RNA samples from mock‐inoculated and virus‐infected plants separated on agarose gel. Arrowhead indicates the accumulation of viral RNAs. (B) Northern blot analyses of virus‐infected and mock‐inoculated plants for AGO1 mRNA at 21 °C. Ethidium bromide‐stained membranes were used as loading control. (C) Western blot of total protein extracts for N . benthamiana  AGO1 or A. thaliana  AGO1 accumulation. Ponceau‐stained membrane was used as loading control. (D) Small RNA Northern blot analyses using miR168‐ and miR159‐specific locked nucleic acid (LNA) probes. Relative gel loadings are also indicated by ethidium bromide staining of ribosomal RNAs (rRNA).