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. 2014 Apr 10;15(7):677–689. doi: 10.1111/mpp.12123

Figure 4.

figure

Interaction and subcellular localization of BnWRKY33, BnMKS1 and BnMPK4 proteins. (a) Interaction of BnWRKY33, BnMKS1 and BnMPK4 in yeast cells. The Gal4 DNA binding domain (BD)‐BnMKS1 fusion bait vectors were co‐transformed with the activation domain (AD)‐BnWRKY33 or BnMPK4 fusion prey vectors into yeast cells, and the transformant cells were assayed for LacZ reporter gene expression on high‐stringency (SD/–Ade/–His/–Leu/–Trp) plates: A, pGBKT7‐BnMKS1 + pGDAT7‐BnWRKY33; B, pGBKT7‐BnMKS1 + pGDAT7‐BnMPK4; C, BnMKS1 (pGBKT7‐BnMKS1) and SV40 large T‐antigen (pGADT7‐T) co‐transformants were used as negative controls. (b) Subcellular localization of BnWRKY33, BnMKS1 and BnMPK4. In planta localization in Nicotiana benthamiana leaves of protein‐green fluorescent protein (GFP), nuclear marker protein‐red fluorescent protein (RFP) and merged fluorescence from RFP and GFP.