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. 2014 Apr 15;15(6):615–626. doi: 10.1111/mpp.12120

Figure 3.

figure

Complementation of yeast strain JO55 with eIF(iso)4E cDNAs. The yeast strain JO55 was transformed with plasmids encoding wild‐type (WT) or mutant eIF(iso)4E [eukaryotic initiation factor(iso)4E]. After transformation, yeast cells were grown in synthetic minimal medium containing 2% glucose. Each yeast clone was serially diluted, and growth on galactose‐ and glucose‐containing media was assessed. Yeasts were incubated at 30 °C for 5 days. WT, susceptible WT Samjin eIF(iso)4E; Gal/Raf, galactose/raffinose; Glu, glucose.