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. 2014 Apr 15;15(6):615–626. doi: 10.1111/mpp.12120

Figure 4.

figure

Turnip mosaic virus (TuMV) screening of T 1 transgenic Chinese cabbage over‐expressing mutated eIF(iso)4E [eukaryotic initiation factor(iso)4E] genes. (a) Polymerase chain reaction (PCR) analysis to confirm the presence of the eIF(iso)4E transgene and hygromycin phosphotransferase II (HPT II) using genomic DNA of T 1 plants that have a single copy of the transgene. M, DNA marker; UT, untransformed control; VT, vector‐transformed control; WT, susceptible wild‐type Samjin eIF(iso)4E transformant; W95L, eIF(iso)4E W95L mutant transformant; W95L/K150E, eIF(iso)4E W95L/K150E transformant. Transgenic T 1 plants selected on the basis of hygromycin (15 mg/mL) resistance were tested. (b) Phenotypes of TuMV CHN5‐inoculated eIF(iso)4E T 1 plants. The plants were photographed at 35 days post‐inoculation (dpi). (c) Enzyme‐linked immunosorbent analysis (ELISA) screening after TuMV inoculation. Double antibody sandwich (DAS)‐ELISA was performed at 35 dpi to assay virus accumulation using the fifth and sixth true leaves. MI, mock‐inoculated control; UT, untransformed ‘Seoul’ wild‐type control; VT, vector‐transformed control. Error bars represent standard deviation. Different letters denote statistically significantly different values [analysis of variance (ANOVA), P ≥ 0.05].