Table 1.
Primer sequences used in the plasmid construction and eIF(iso)4E site‐directed mutagenesis
| Primer ID | Primer sequence | Direction |
|---|---|---|
| TuMV VPg (Nco1) | CCATGGATGGCGAAAGGCAAGAGGCA | F |
| TuMV VPg (Sal1) | GTCGACCTATCGTGGTCCACTGGGAC | R |
| eIF(iso)4E (EcoR1) | GAATTCATGGCGACAGAGGATGTGAA | F |
| eIF(iso)4E (Xho1) | CTCGAGTCAGACAGTGAACCGAGTTC | R |
| W49L | CAAGGCGCCGCCCTTGGAGCCTCCCTTCG | F |
| CGAAGGGAGGCTCCAAGGGCGGCGCCTTG | R | |
| W95L | GTTGAGCCTAAGCTTGAAGATCCTGAG | F |
| CTCAGGATCTTCAAGCTTAGGCTCAAC | R | |
| K105L | GCTAATGGCGGACTTTGGACTTTTGTT | F |
| AACAAAAGTCCAAAGTCCGCCATTAGC | R | |
| T107L | GGCGGAAAGTGGCTTTTTGTTGTTACC | F |
| GGTAACAACAAAAAGCCACTTTCCGCC | R | |
| K150L | AGTGTGCGCCCACTTCAGGACAAGCTC | F |
| GAGCTTGTCCTGAAGTGGGCGCACACT | R | |
| D191L | ACTAACCATGATCTTTCTAGAAGAACT | F |
| AGTTCTTCTAGAAAGATCATGGTTAGT | R | |
| S192L | AACCATGATGATCTTAGAAGAACTCGG | F |
| CCGAGTTCTTCTAAGATCATCATGGTT | R | |
| RT‐PCR eIF#1 | CTTCTGGGGTTTGCACGA | F |
| CCGACAAGAGCCATCAAAGTTTC | R | |
| HPT2 | CCTGAACTCACCGCGACG | R |
| AAGACCAATGCGGAGCATAT | F | |
| Brassica actin | GTGACAATGGAACTGGAATGG | F |
| AGACGGAGGATAGCGTGAGG | R |
eIF(iso)4E, eukaryotic initiation factor(iso)4E; HPT, hygromycin phosphotransferase; RT‐PCR, reverse transcription‐polymerase chain reaction; TuMV, Turnip mosaic virus; VPg, viral protein genome‐linked.