Table 2.
Primer sequences used in real‐time reverse transcription‐polymerase chain reaction (RT‐PCR)
| Primer ID | Primer sequence | Direction | Target |
|---|---|---|---|
| Bra(iso)4E_3UTR | ACGGTCTTGGATGGTGGTTG | F | Endogenous eIF(iso)4E |
| GGCAGTCGGTCATACCTGTG | R | ||
| Bra_(iso)4E_RTc | AGCCTGCTGACAAGCTCGAA | F | eIF(iso)4E transgene |
| CCACTTTCCGCCATTAGCAC | R | ||
| Bra_eIF4E_RTb | TCCGTCTTCACCTTCTCCAC | F | Endogenous |
| CCAATCAAAGCAAGCAAGGT | R | eIF4E | |
| Bra‐actin‐b | TTCAATGTCCCTGCCATGTA | F | Brassica actin |
| CTCGGCAGTAGTGGTGAACA | R |
eIF(iso)4E, eukaryotic initiation factor(iso)4E.