Changes in expression of Mf2/2, cpst12 and pro1 in the CpSte11 null mutants compared with the wild‐type (EP155/2), hypovirulent (UEP1), Cpmk1 and Cpmk2 mutant strains. Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of Mf2/2 (A), cpst12 (B) and pro1 (C) transcript levels relative to levels of glyceraldehyde‐3‐phosphate dehydrogenase (gpd). The values on the y‐axis were normalized to the transcript levels of the corresponding gene in 5‐day cultured EP155/2 strain, with standard deviations, based on three independent measurements of two independent RNA preparations, indicated by the error bars.