Figure 3.

Identification of evolutionarily conserved regions in BcSpl1. (A) Conservation indices (grey bars) were calculated with AL2CO for every BcSpl1 residue from the alignment of either 146 complete cerato‐platanin family sequences downloaded from Pfam (top panel) or the eight cerato‐platanins known (Buensanteai et al., 2010; Comparini et al., 2009; Frías et al., 2011; Pazzagli et al., 1999; Vargas et al., 2008; Wilson et al., 2002; Yang et al., 2009; Zaparoli et al., 2009) to elicit plant defences (bottom panel). Zero corresponds to the average conservation index for the whole protein. Average conservation indices were also calculated for windows of five residues for the whole protein (thin line) or after deleting the four cysteines (thick line) to avoid the effect of its absolute conservation. Broken line boxes indicate the five regions with conservation indices well above average (zero) in the two alignments. (B) Position of the five conserved regions in the structure of BcSpl1 predicted by SWISS‐MODEL in three different views.