Figure 5.
Virulence assay on host tobacco plants. Tobacco leaves were inoculated with each bacterium by the infiltration method at 2 × 105 colony‐forming units (cfu)/mL (A) and by the dip inoculation method at 2 × 108 cfu/mL (B), and were photographed at 9 and 12 days post‐inoculation (DPI), respectively. (C) Bacterial populations were measured at 1, 3 and 6 DPI by the dip inoculation method using three tobacco leaf discs (8 mm in diameter). The bars represent standard deviations for at least three independent experiments. Asterisks indicate a significant difference from the wild‐type (WT) in a t‐test (*P < 0.05, **P < 0.005).