Table 2.
Bacterial strains and plasmids used in this study.
| Bacterial strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Escherichia coli strain | ||
| DH5a | F–λ–ø80dLacZΔM15 Δ(lacZYA‐argF)U169 recA1 endA1 hsdR17 (rK–mK+) supE44 thi‐1gyrA relA1 | Takara, Kyoto, Japan |
| S17‐1 | thi pro hsdR–hsdM + recA [chr::RP4‐2‐Tc::Mu‐Km::Tn7] | Schäfer et al. (1994) |
| S17‐1 λ‐pir | λ pir lysogen of S17 (Tpr Smr thi pro hsdR‐M+recA RP::2‐Tc::Mu‐Km::Tn7 | Simon et al. (1983) |
| Pseudomonas syringae pv. tabaci | ||
| Isolate 6605 | Wild‐type, Nalr | Taguchi et al. (2001) |
| 6605‐ΔpilA | Isolate 6605 ΔpilA, Nalr | Taguchi and Ichinose (2011) |
| 6605‐ΔtfpO | Isolate 6605 ΔtfpO, Nalr | This study |
| 6605‐ΔtfpO‐C | Isolate 6605 ΔtfpO complemented, Nalr (pBSLtfpO) | This study |
| Isolate 6823 | Wild‐type | Taguchi et al. (2001) |
| Isolate 7375 | Wild‐type | Taguchi et al. (2001) |
| Isolate MD340a | Wild‐type | Gift from Dr J. Lydon |
| Isolate 113R | Wild‐type | Gift from Dr J. Lydon |
| Isolate ATCC 11528 | Wild‐type | Rico and Preston (2008) |
| Plasmid | ||
| pCR‐Blunt II‐TOPO | Cloning vector, Kmr | Invitrogen |
| pTOPO‐pilA | 5533‐bp insert containing pilA surrounding, Kmr | This study |
| pGEM‐T easy | Cloning vector, Ampr | Promega, Madison, WI, USA |
| pGEM‐tfpO | 3704‐bp insert containing pilA and tfpO, Ampr | This study |
| pGEM‐ΔtfpO | 3204‐bp insert containing pilA and 500‐bp interval deleted tfpO, Ampr | This study |
| pGEM‐tfpO‐C | 2339‐bp insert containing pilA and tfpO, Ampr | This study |
| pK18mobsacB | Small mobilizable vector, Kmr, sucrose‐sensitive (sacB) | Schäfer et al. (1994) |
| pK18‐ΔtfpO | 3204‐bp insert through EcoRI site containing pilA and 500‐bp interval deleted tfpO, Kmr | This study |
| pBSL118 | Mini‐Tn5 derived plasmid vector for insertion mutagenesis, Ampr, Kmr | Alexeyev et al. (1995) |
| pBSLtfpO‐C | 2339‐bp insert containing pilA and tfpO, Ampr, Kmr | This study |
Ampr, ampicillin resistance; Kmr, kanamycin resistance; Nalr, nalidixic acid resistance.