Table 2.

Site‐directed mutagenesis: overview of the constructed and inoculated mutants

Mutant name	Primers 5′–3′	Codon From–to	AA change	Symptoms on tomato*
P‐22flc‐CPE155K	TTGGGCTATCAGAAAGATACCAAG	155	E–K	IY
	TTTTGCCCAGTTGGCAGGTGGCAC	GAA–AAA
P‐22flc‐CPE155R	TTGGGCTATCAGAGAGATACCAAG	155	E–R	Yellow dots
	TTTTGCCCAGTTGGCAGGTGGCAC	GAA–AGA
P‐22flc‐CPE155A	TTGGGCTATCAGGCAGATACCAAGT	155	E–A	None
	TTTTGCCCAGTTGGCAGGTGGCAC	GAA–GCA
P‐22flc‐CPE155D	GGGCTATCAGGATGATACCAAGT	155	E–D	None
	AATTTTGCCCAGTTGGCAGGTGGC	GAA–GAT
P‐22flcCPD166G	TGACTTCTTTGGTGGAGTCACAAA	166	D–G	IY
	AGAGCAGCAAACTTGGTATCTTCC	GAT–GGT
P‐5flc‐CPK155E	TTGGGCTATCAGGAAGATACCAAG	155	K–E	None
	TTTTGCCCAGTTGGCAGGTGGCAC	AAA–GAA
P‐5flc‐CPK155R	TTGGGCTATCAGAGAGATACCAAG	155	K–R	Yellow dots
	TTTTGCCCAGTTGGCAGGTGGCAC	AAA–AGA
P‐5flc‐CPK155A	TTGGGCTATCAGGCAGATACCAAGT	155	K–A	None
	TTTTGCCCAGTTGGCAGGTGGCAC	AAA–GCA
P‐22flc‐CPS8A	ACCTACAGCTGCTAACCCCATC	8	S–A	None
	TGGTTTTCCATAGTTGAGTTA	TCT–GCT
P‐22flc‐CPV43I	GATTTGAAGAAGGTCAAATACGTG	43	I–V	None
	ACTTAGGGAAGGAGCTGTATTAG	ATC–GTC
P‐5flc‐CPS9N	CTACAGCTTCTAACCCATCAGATGC	9	S–N	None
	GTTGGTTTTCCATAGTTGAGTTAA	AGC–AAC

The italic letters indicate the codons that were introduced.

AA, amino acid.

*IY, interveinal leaf yellowing.