Table 1.
Strains and plasmids used in this study
| Strains or plasmids | Relevant characteristics | Reference or source |
|---|---|---|
| Xanthomonas oryzae pv. oryzae | ||
| PXO99A | Wild‐type, Philippine race 6 | This laboratory |
| PΔhrcU | hrcU deletion mutant of strain PXO99A | Li et al. (2011b) |
| Xanthomonas oryzae pv. oryzicola | ||
| RS105 | Wild‐type, Chinese race 2; Rifr | Zou et al. (2006) |
| RΔhrpE3 | hrpE3 deletion mutant of strain RS105; Rifr | This work |
| CRhrpE3 | RΔhrpE3 containing pP2HrpE3‐c‐Myc; Rifr Kmr | This work |
| CRhrpE3(201hpaE) | RΔhrpE3 containing pP2HpaE‐c‐Myc; Rifr Kmr | This work |
| RΔhrcC | hrcC deletion mutant of strain RS105; Rifr | Li et al. (2011a) |
| RΔhrpG | hrpG deletion mutant of strain RS105, Rifr | Jiang et al. (2009a) |
| RΔhrpX | hrpX deletion mutant of strain RS105; Rifr | Jiang et al. (2009a) |
| RΔhpaB | hpaB deletion mutant of strain RS105, Rifr | Li et al. (2011b) |
| RΔhrcV | hrcV deletion mutant of strain RS105; Rifr | This laboratory |
| RΔhrpD5 | hrpD5 deletion mutant of strain RS105, Rifr | Li et al. (2011a) |
| RΔhrpD6 | hrpD6 deletion mutant of strain RS105, Rifr | Li et al. (2011a) |
| RΔhrpE | hrpE deletion mutant of strain RS105, Rifr | Li et al. (2011a) |
| Xanthomonas campestris pv. vesicatoria | ||
| 23‐1 | Wild‐type, Chinese race | Laboratory collection |
| Escherichia coli | ||
| DH5α |
F− Φ80dlacZ ΔM15Δ (lacZYA‐argF)U169 endA1 deoR recA1 hsdR17(rK
− mK+) phoA supE44
λ− thi‐l gyrA96 relA1 |
Clontech |
| Agrobacterium tumefaciens | ||
| EH105 | Rifr | This laboratory |
| Plasmids | ||
| pMD18‐T | pUC ori, cloning vector; Apr | TaKaRa |
| pUFR034 | IncW, Mob(p), Mob −, LacZa +, PK2 replicon, cosmid; Kmr | De Feyter et al., 1990 |
| pKMS1 | 6.4‐kb derivative from pK18mobGII, sacB +; Kmr | Zou et al., 2011 |
| pKΔhrpE3 | 1250‐bp fusion ligated in pKMS1 with a 285‐bp deletion in hrpE3; Kmr | This work |
| pP1HrpE3‐c‐Myc | pUFR034 expressing hrpE3 under the control of its own 397‐bp promoter with a c‐Myc tag; Kmr | This work |
| pP2HrpE3‐c‐Myc | pUFR034 expressing hrpE3 under the control of its own 201‐bp promoter with a c‐Myc tag; Kmr | This work |
| pP3HrpE3‐c‐Myc | pUFR034 expressing hrpE3 under the control of its own 101‐bp promoter with a c‐Myc tag; Kmr | This work |
| p987HpaE‐c‐myc | pUFR034 expressing hpaE under the control of its own 987‐bp promoter with a c‐Myc tag; Kmr | This work |
| pP1HpaE‐c‐myc | pUFR034 expressing hpaE under the control of hrpE3 398‐bp promoter with a c‐Myc tag; Kmr | This work |
| pP2HpaE‐c‐myc | pUFR034 expressing hpaE under the control of hrpE3 201‐bp promoter with a c‐Myc tag; Kmr | This work |
| pP2nE3‐cE | pUFR034 expressing the fusion encoding the 50 N‐terminal amino acids of HrpE3 and the last 44 C‐terminal amino acids of HpaE under a 201‐bp hrpE3 promoter; Kmr | This work |
| pP1GUS | pUFR034 expressing GUS under the 397‐bp promoter of hrpE3; Kmr | This work |
| pP2GUS | pUFR034 expressing GUS under the 201‐bp promoter of hrpE3; Kmr | This work |
| pP3GUS | pUFR034 expressing GUS under the 101‐bp promoter of hrpE3; Kmr | This work |
| p987GUS | pUFR034 expressing GUS under the 987‐bp promoter of hpaE; Kmr | This work |
| p397GUS | pUFR034 expressing GUS under the 397‐bp promoter of hpaE; Kmr | This work |
| p201GUS | pUFR034 expressing GUS under the 201‐bp promoter of hpaE; Kmr | This work |
| p101GUS | pUFR034 expressing GUS under the 101‐bp promoter of hpaE; Kmr | This work |
| pavrXa10 | pUFR034 expressing AvrX10, Kmr | Li et al. (2011b) |
| pavrXa10Δ | pUFR034 expressing AvrXa10Δ, Kmr | Li et al. (2011b) |
| pP2avrXa10Δ | avrXa10Δ expression from the 205‐bp promoter of hrpE3 in pUFR034; Kmr | This work |
| pP1E3avrXa10Δ | The first 50 amino acids of HrpE3 fused with AvrXa10Δ under a 397‐bp promoter region of hrpE3 in pUFR034; Kmr | This work |
| pP2E3avrXa10Δ | The first 50 amino acids of HrpE3 fused with AvrXa10Δ under a 201‐bp promoter region of hrpE3 in pUFR034; Kmr | This work |
| pP3E3avrXa10Δ | The first 50 amino acids of HrpE3 fused with AvrXa10Δ under a 101‐bp promoter region of hrpE3 in pUFR034; Kmr | This work |
| pP2EavrXa10Δ | The first 50 amino acids of HpaE fused with AvrXa10Δ under a 201‐bp promoter region of hrpE3 in pUFR034; Kmr | This work |
| pEGAD | lacZa+, contains GFP; Kmr | This laboratory |
| pHrpE3‐GFP | A 285‐bp hrpE3 was fused in frame with gfp in vector pEGAD; Kmr | This work |
| pHpaE‐GFP | A 300‐bp hpaE was fused in frame with gfp in vector pEGAD; Kmr | This work |
| pXopR‐GFP | A 1314‐bp XopR was fused in frame with gfp gene in vector pEGAD; Kmr | Guo et al. (2012) |
Apr, ampicillin resistance; GFP, green fluorescent protein; GUS, β‐glucuronidase; Kmr, kanamycin resistance; Rifr, rifampicin resistance.