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. 2012 Feb 1;13(7):719–731. doi: 10.1111/j.1364-3703.2011.00780.x

Table 1.

Materials and method of preparation of cDNA for transcriptome sequencing. The contribution of each pool to the number of reads and number of bases is indicated.

Pool Material Strategy 5′ reads* Total 5′ nucleotides 3′ reads Total 3′ nucleotides
1 Spores 5′ sequencing 86 352 22 212 388
2 Interaction 5′ sequencing 115 413 30 335 206
3 Interaction Random primed + normalized 701 499 182 312 369
4 Mixed Random primed + normalized 637 709 165 624 293
3+4 Mixed Random primed + normalized 1 013 411 262 824 074
*

The DNA code in the adapter that links a read to a pool could not be resolved for 19 422 reads that are not included in this table.

The DNA code was not sequenced in 3′ reads.