Table 1.
Materials and method of preparation of cDNA for transcriptome sequencing. The contribution of each pool to the number of reads and number of bases is indicated.
Pool | Material | Strategy | 5′ reads* | Total 5′ nucleotides | 3′ reads | Total 3′ nucleotides |
---|---|---|---|---|---|---|
1 | Spores | 5′ sequencing | 86 352 | 22 212 388 | – | – |
2 | Interaction | 5′ sequencing | 115 413 | 30 335 206 | – | – |
3 | Interaction | Random primed + normalized | 701 499 | 182 312 369 | – | – |
4 | Mixed | Random primed + normalized | 637 709 | 165 624 293 | – | – |
3+4† | Mixed | Random primed + normalized | – | – | 1 013 411 | 262 824 074 |
The DNA code in the adapter that links a read to a pool could not be resolved for 19 422 reads that are not included in this table.
The DNA code was not sequenced in 3′ reads.