Table 3.

Expression profile of genes involved in metabolism pathway of Candidatus  Liberibacter asiaticus in planta compared with in psyllid

Locus tag	Gene nameb	Gene productc	KEGG pathway	Relative expressiond
Carbohydrate metabolism
CLIBASIA_00375	fumC	Fumarate hydratase	Citrate cycle	3.39
CLIBASIA_00825	glk	Glucokinase	Glycolysis	2.78
CLIBASIA_01065	NA	UDP‐glucose 4‐epimerase	Galactose	3.44
CLIBASIA_01165	NA	5‐Amino‐6‐(5‐phosphoribosylamino)uracil reductase/diaminohydroxyphosphoribosylaminopyrimidine	Riboflavin metabolism	3.88
CLIBASIA_01680	acnA	Aconitate hydratase	Glyoxylate, dicarboxylate metabolism/citrate cycle	5.27
CLIBASIA_02695	NA	Fructose‐bisphosphate aldolase	Glycolysis	3.4
CLIBASIA_02700	pgk	Phosphoglycerate kinase	Glycolysis	2.1
CLIBASIA_02705	NA	Glyceraldehyde 3‐phosphate dehydrogenase	Glycolysis	2.77
CLIBASIA_02710	tkt	Transketolase	Pentose phosphate pathway	6.24
CLIBASIA_02785	eno	Phosphopyruvate hydratase	Glycolysis	2.46
CLIBASIA_02810	lpdA	Dihydrolipoamide dehydrogenase	Glycolysis	1.49
CLIBASIA_03595a	pckA	Phosphoenolpyruvate carboxykinase	Glycolysis	0.79
CLIBASIA_04750	mdh	Malate dehydrogenase	Glyoxylate,dicarboxylate metabolism	2.32
CLIBASIA_05045	NA	Phosphoglucomutase	Glycolysis	3.47
Energy metabolism
CLIBASIA_00340	glnA	Glutamine synthetase protein	Alanine, aspartate and glutamate metabolism	4.76
CLIBASIA_00345	glsA	Glutaminase	d‐glutamine and d‐glutamate metabolism	1.76
CLIBASIA_00585a	NA	Inorganic pyrophosphatase	Oxidative phosphorylation	2.2
CLIBASIA_02750	NA	Carbonate dehydratase	Nitrogen metabolism	1.33
CLIBASIA_03735	nuoA	NADH dehydrogenase subunit A	Oxidative phosphorylation	−2.09
CLIBASIA_04725	sdhA	Succinate dehydrogenase flavoprotein subunit	Oxidative phosphorylation	2.47
Nucleotide metabolism
CLIBASIA_00055	gmk	Guanylate kinase	Purine metabolism	2.41
CLIBASIA_00400	pyrG	CTP synthetase		1.51
CLIBASIA_00515	tmk	Thymidylate kinase	Pyrimidine metabolism	3.87
Metabolism of terpenoids and polyketides
CLIBASIA_03210	ispB	Octaprenyl‐diphosphate synthase protein	Terpenoid backbone biosynthesis	2.23
CLIBASIA_04105	rfbA	Glucose‐1‐phosphate thymidylyltransferase	Polyketide sugar unit biosynthesis	1.82
CLIBASIA_05065	NA	Geranyltranstransferase protein	Terpenoid backbone biosynthesis	3.65
Metabolism of cofactors and vitamins
CLIBASIA_00425	hemH	Ferrochelatase	Porphyrin and chlorophyll metabolism	3.29
CLIBASIA_01160	NA	Riboflavin synthase subunit α	Riboflavin metabolism	−2.99
CLIBASIA_01965	folD	Methylenetetrahydrofolate dehydrogenase/cyclohydrolase protein	One‐carbon pool by folate	1.9
CLIBASIA_02815	NA	Lipoyl synthase	Lipoic acid metabolism	2.84
CLIBASIA_03515	hemE	Uroporphyrinogen decarboxylase	Porphyrin and chlorophyll metabolism	3.93
CLIBASIA_03560	folC	FolC bifunctional protein	Folate biosynthesis	2.49
CLIBASIA_04185	acpS	4′‐Phosphopantetheinyl transferase	Pantothenate and CoA biosynthesis	1.84
CLIBASIA_04670	NA	Hypothetical protein	Folate biosynthesis	2.65
CLIBASIA_04685	hemD	Uroporphyrinogen‐III synthase	Porphyrin and chlorophyll metabolism	3.03
CLIBASIA_04695	hemC	Porphobilinogen deaminase	Porphyrin and chlorophyll metabolism	4.1
CLIBASIA_04875	NA	Coproporphyrinogen III oxidase	Porphyrin and chlorophyll metabolism	2.16
Amino acid metabolism
CLIBASIA_04835	kamA	l‐lysine 2,3‐aminomutase protein	Lysine degradation	6.39
CLIBASIA_02600	NA	Creatinine amidohydrolase	Arginine/proline metabolism	8.43
CLIBASIA_00220	NA	Phytoene synthase protein	Biosynthesis of secondary metabolites	8.56
CLIBASIA_03995	thrA	Homoserine dehydrogenase	Glycine, serine and threonine metabolism	2.30
CLIBASIA_01170	glyA	Serine hydroxymethyltransferase	Glycine, serine and threonine metabolism	2.32
CLIBASIA_04210	glmS	Glucosamine‐fructose‐6‐phosphate aminotransferase	Alanine, aspartate and glutamate metabolism	−1.71
Metabolism of other amino acids
CLIBASIA_02780	kdsA	2‐Dehydro‐3‐deoxyphosphooctonate aldolase	Lipopolysaccharide biosynthesis	1.82
CLIBASIA_02915	gshB	Glutathione synthetase	Glutathione metabolism	1.59
CLIBASIA_03015	gnd	6‐Phosphogluconate dehydrogenase	Glutathione metabolism	7.01
CLIBASIA_03280	kdsB	3‐Deoxy‐manno‐octulosonate cytidylyltransferase	Lipopolysaccharide biosynthesis	3.29
CLIBASIA_03290	lpxB	Lipid‐A‐disaccharide synthase	Lipopolysaccharide biosynthesis	6.12
CLIBASIA_03420	mviN	Integral membrane protein MviN	Peptidoglycan biosynthesis	3.62
CLIBASIA_04785	gor	Glutathione reductase	Glutathione metabolism	4.17
Genetic information processing
Folding, sorting and degradation
CLIBASIA_03720	NA	Chaperonin GroEL	RNA degradation	1.85
Replication and repair
CLIBASIA_02530	NA	Primosome assembly protein PriA	Homologous recombination	4.97

^aPsyllid RNA samples were extracted from a single psyllid (see Experimental procedures).

^bNA, gene name was not assigned.

^cInformation on gene product was based on the annotation of the genome of Ca. L. asiaticus strain psy62 and definition in the KEGG database.

^dFold change (log₂ ratio) is the relative gene expression (in planta versus in psyllid) of Ca. L. asiaticus. Positive value indicates that gene was overexpressed in planta compared with in psyllid; negative value indicates that gene was overexpressed in psyllid compared with in planta.