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. 2013 Oct 28;15(2):196–210. doi: 10.1111/mpp.12080

Figure 1.

figure

Affinities between Bamboo mosaic virus (BaMV) coat protein (CP) and various regions of BaMV RNA 5′ untranslated region (UTR). (A) The sequence and computer‐predicted (Zuker, 2003) secondary structure of radiolabelled probes of BaMV used in this figure. (B) Electrophoresis mobility shift assay (EMSA) was used to locate the RNA regions in the 5′ UTR required for interaction with BaMV CP. For each reaction, 1 fmol of radiolabelled probe, corresponding to nucleotides 1–93, 1–52, 27–69 or 54–93 in the 5′ UTR of BaMV RNA (as indicated at the top), was incubated either alone (–) or in the presence (+) of wild‐type (wt) BaMV CP (2 μm) for 10 min, followed by electrophoresis through a 1% agarose gel. The signals were analysed by the BAS‐2500 imaging system (Fujifilm). The position of the free probe is indicated on the right.