Figure 4.

Effects of coat protein (CP) mutations on RNA binding. (A) The ³²P‐labelled probe (1 fmol), representing nucleotides 1–52 of Bamboo mosaic virus (BaMV) RNA 5′ untranslated region (UTR), was incubated with 2 μm (1×) or 6 μm (3×) of various CPs as indicated at the top. Bovine serum albumin (BSA) (2 μm) was used as a negative control. The relative amounts (%) of free probe are shown at the bottom. (B) The ³²P‐labelled probe (0.1 fmol) of CP subgenomic RNA of BaMV (nucleotides 5389–6366) or Tobacco mosaic virus (TMV) (nucleotides 5546–6392) was incubated with 1 μm of various CPs as indicated at the top. The samples were analysed by electrophoresis through a 5% (A) or 3.5% (B) polyacrylamide gel in 0.5 × TBE, and signals were detected by autoradiography. The positions of the free probe and shifted complexes with slower mobilities are indicated on the right. wt, wild‐type.