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Southern blotting of 38 Xanthomonas oryzae pv. oryzae (Xoo) strains. Genomic DNA of Xoo strains was digested with SphI and Sau3 AI, separated in 1.2% (w/v) agarose gel and transferred to a nylon membrane. The SphI ‐Sau3 AI fragment containing the central repeat region of avrXa23 was labelled with 32 P‐dCTP and used as a DNA probe. M, molecular ladder; CK, the 2797‐bp SphI ‐Sau3 AI fragment of avrXa23; 1–38, numbers of the 38 Xoo strains in Table 1; CK2, P99ΔC9.
