Figure 1.

Early and late regulation of defence‐related genes during rice blast infection. Gene expression was measured by reverse transcription‐quantitative polymerase chain reaction in plants inoculated with gelatin only (mock treatment) or with Magnaporthe oryzae (virulent isolate FR13, grey bars; avirulent isolate CL3.6.7, black bars) at different time points after treatment. Gene expression was normalized using actin. The results are the log₂ values of the ratio of the mean transcript levels for inoculated vs. mock‐treated plants from four independent biological replicates. A t‐test was performed to establish whether the expression of one given gene inoculated with the virulent or avirulent isolate was different from its corresponding expression in the mock‐treated plants (plain black or grey bars, P < 0.05; no statistically significant difference, hatched bars).