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. 2011 Jun 1;13(1):58–71. doi: 10.1111/j.1364-3703.2011.00726.x

Figure 1.

Figure 1

Growth curve and extracellular protease (Prt) activity of Pectobacterium carotovorum strains. (a) Prt activity produced by P. carotovorum strains on nutrient gelatin (NG) agar medium. Bacteria were patched on NG agar plates and incubated at 28 °C overnight. Prt activity was determined visually by the production of a halo around the colony: 1, parent KD100 (top left); 2, CorA‐ mutant KD101 (top right); 3, parent Ecc71 (bottom left); 4, CorA‐ mutant KD102 (bottom right). (b) Growth curves of parents KD100 and Ecc71 and CorA‐ mutants KD101 and KD102. Bacteria were grown in minimal salts (MM) medium at 28 °C and growth was monitored hourly by Klett meter.