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. 2011 Jul 4;13(2):123–134. doi: 10.1111/j.1364-3703.2011.00735.x

Figure 1.

Figure 1

The detached cotyledons of the hp1 mutant are hypersusceptible to nontumorigenic Agrobacterium tumefaciens infection. (A) Necrosis on cotyledons caused by A. tumefaciens GV2260. Cotyledons detached from wild‐type (WT) AC+ and hp1 mutant seedlings, germinated on half‐strength Murashige and Skoog (MS) medium, were inoculated with A. tumefaciens GV2260 carrying the binary vector pBI121 (containing the kanamycin resistance gene) and incubated on regeneration medium under 1000 lx. Photographs were taken 10 and 30 days after infection. (B) Quantification of kanamycin‐resistant callus produced on the cotyledons. Each data point consists of at least three samples. Error bars indicate standard deviation. The statistical significance of the difference was confirmed by Student's t‐test (**P < 0.01). Similar results were obtained in at least two independent experiments.