Figure 3.

Agrobacterium‐mediated transient transformation in the leaves of wild‐type (WT) AC⁺ and hp1 mutant plants. (A) Randomly selected representative leaves of WT AC⁺ and hp1 mutant plants stained with 5‐bromo‐4‐chloro‐3‐indolyl glucuronide (X‐Gluc) at 2 days after infiltration with A. tumefaciens GV2260 expressing the β ‐glucuronidase (GUS) gene at an inoculum of OD₆₀₀ = 0.1 (optical density at 600 nm). Bars, 1 cm. (B) Quantification of GUS activity in WT AC⁺ and hp1 mutant leaves. The GUS activity of infected leaves was measured by recording the fluorescence of 4‐methylumbelliferone (MU) at 2 days post‐inoculation (dpi). The data presented are the means with standard deviations of three independent experiments. The statistical significance of the difference was confirmed by Student's t‐test (**P < 0.01).