Figure 4.

Effect of HP1/DDB1 on the induction of SlPR1a1 (A), SlPti5 (B), SlLrr22 (C), SlGras2 (D) and SlWrky28 (E) by Agrobacterium tumefaciens. Six‐week‐old wild‐type (WT) AC⁺ and hp1 mutant plants were vacuum infiltrated with A. tumefaciens GV2260 [optical density at 600 nm (OD₆₀₀) = 0.1]. Total RNA was extracted at the indicated time points for quantitative reverse transriptase‐polymerase chain reaction (RT‐PCR). SlUBI3 was used as an internal control. Data represent the mean ± standard deviation of three independent experiments. The statistical significance of the difference was confirmed by Student's t‐test (*P < 0.05; **P < 0.01). hpi, hours post‐inoculation.