Figure 5.

Influence of salicylic acid (SA) treatment on SlPR1a1 expression and the susceptibility of the hp1 mutant to Agrobacterium tumefaciens. (A) SlPR1a1 expression in wild‐type (WT) AC⁺ and hp1 mutant after SA treatment. Leaf samples sprayed with 0.5 mm SA solution were collected for quantitative reverse transriptase‐polymerase chain reaction (RT‐PCR) analysis at the indicated time points. SlUBI3 was used as an internal control. Data represent the mean ± standard deviation from three independent experiments. The statistical significance of the difference was confirmed by Student's t‐test (**P < 0.01). (B) Bacterial growth in infected leaves of WT AC⁺ and hp1 mutant plants treated with SA. Leaves were presprayed with 1 mm SA or mock solution, 48 h before inoculation with A. tumefaciens GV2260 at an inoculum of 1 × 10⁴ colony‐forming units (cfu)/mL [optical density at 600 nm (OD₆₀₀) = 0.00001]. Bacterial growth was determined at 0, 2 and 4 days post‐inoculation (dpi). Each data point consists of at least six samples. Error bars indicate standard deviation. The statistical analysis of the difference between the hp1 mutant and WT AC⁺ for both buffer and SA treatment was confirmed by Student's t‐test (**P < 0.01). Similar results were obtained in at least two independent experiments.