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. 2013 Mar 25;14(6):543–556. doi: 10.1111/mpp.12026

Figure 3.

figure

(A,B) Assays for the sensitivity of the EV‐MIL31 strain (wild‐type, WT), the strains carrying a single deletion of NoxA, NoxB or NoxR, the rescued strains (CpB24 and CpR40) and the NoxA NoxB (ΔnoxAB) and NoxA NoxR (ΔnoxAR) double‐mutant strains of Alternaria alternata. Two independent mutant strains of each type were used for the assays. Fungi were grown on potato dextrose agar (PDA) amended with the test compounds for 4–6 days. Radial growth was measured. The percentage change in radial growth was calculated as the percentage of growth of the deletion mutants in relation to the wild‐type grown on the same plate. A negative percentage change indicates growth reduction in relation to the wild‐type. The mutant was considered to be hypersensitive to the test compounds when the percentage change in growth reduction was greater than that measured in untreated PDA. The data presented are the mean and standard error of two independent mutants with at least two replicates of two experiments (n = 8). For each test compound, means indicated by the same letter within a test compound are not significantly different from one another, P < 0.05. RB, rose bengal; MND, menadione.