Influence of heterologous expression of ZmRop1 on Potato virus X (PVX) infection in Nicotiana benthamiana. (A) Symptoms caused by PVX infection in ZmRop1, ZmRop1CA and ZmRop1DN heterologous expression and control plants were photographed at 12 days post‐inoculation (dpi). CA, constitutively active, DN, dominant negative. (B) Analysis of PVX accumulation by Western blotting with PVX coat protein (CP) antibody, as indicated. The Coomassie brilliant blue‐stained 8% sodium dodecylsulphate‐polyacrylamide gel (bottom) for different samples was used as a loading control.