Table 1.
Comparison of mitogen‐activated protein kinase kinase (MAP2K)‐null mutants, complemented strains and wild‐type isolate (Δcpku80). Reported values are means ± standard deviation. Data followed by the same letter are not significantly different according to Tukey's test (P < 0.05). Colony diameter was taken after 7 days of growth
| Characteristic | Δcpkk1 | Δcpkk2 | Δcpkk3 | Δcpku80 | Δcpkk1‐compl | Δcpkk2‐compl |
|---|---|---|---|---|---|---|
| Growth on PDAmb (cm) | 2.69 ± 0.23a | 1.95 ± 0,30b | 7.23 ± 0.41c | 7.42 ± 0.60c | 7.23 ± 0.35c | 7.59 ± 0.31c |
| Biomass dry weight (g) from liquid culture | 0.07 ± 0.01a | 0.27 ± 0.03b | 0.28 ± 0.01b | 0.28 ± 0.02b | 0.29 ± 0.04b | 0.27 ± 0.01b |
| Growth on PDAmb + 2 m sorbitol (cm) | 1.64 ± 0.14a | 1.21 ± 0.18b | No | 5.95 ± 0.30c | 5.77 ± 0.12c | 5.86 ± 0.16c |
| Growth on PDAmb + 4% NaCl (cm) | 0.75 ± 0.41a | 1.05 ± 0.11b | No | 2.00 ± 0.10c | 1.88 ± 0.14c | 1.97 ± 0.11c |
| Growth on PDAmb + 0.125 mm menadione (cm) | 0.76 ± 0.09a | 0.89 ± 0.13a | 2.33 ± 0.15b | 2.41 ± 0.10b | 2.18 ± 0.17b | 2.27 ± 0.20b |
| Growth on PDAmb + 0.005% SDS (cm) | No | 0.68 ± 0.10a | 2.86 ± 0.33b | 4.68 ± 0.34c | 4.24 ± 0.54c | 4.61 ± 0.27c |
| Growth on PDAmb + 100 mm H2O2 (cm) | No | 0.86 ± 0.12a | 5.19 ± 0.32b | 5.02 ± 0.23b | 4.95 ± 0.31b | 5.05 ± 0.27b |
| Asexual sporulation (conidiospores × 107/mL) | No | No | 2.91 ± 0.15a | 2.72 ± 0.25a | 2.34 ± 0.18a | 2.47 ± 0.11a |
| Stromal pustule production | No | No | Yes | Yes | Yes | Yes |
| Canker area on chestnut at 21 days post‐inoculation (cm2) | 0.27 ± 0.14a | 0.21 ± 0.06a | 2.91 ± 0.69b | 3.12 ± 0.58b | 2.98 ± 0.58b | 2.96 ± 0.54b |
PDAmb, potato dextrose agar (PDA) containing l‐methionine (100 mg/L) and biotin (1 mg/L); SDS, sodium dodecylsulphate.