Table 1.
Azole sensitivities of Pyrenopeziza brassicae isolates carrying particular PbCYP51 variants
| PbCYP51 variant | Fungicide | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tebuconazole | Metconazole | Flusilazole | Prothioconazole | Prochloraz | ||||||
| Mean EC50 (Range) (μg/mL) | RFa | Mean EC50 (Range) (μg/mL) | RF | Mean EC50 (Range) (μg/mL) | RF | Mean EC50 (Range) (μg/mL) | RF | Mean EC50 (Range) (μg/mL) | RF | |
|
Wild‐type (n b = 14) |
0.04 (0.01–0.15) |
1 |
0.02 (0.004–0.09) |
1 |
0.07 (0.01–0.20) |
1 |
0.14 (0.02–0.79) |
1 |
0.03 (0.01–0.08) |
1 |
|
G460S (n = 8) |
0.49 (0.23–0.79) |
12 |
0.15 (0.10–0.22) |
8 |
0.68 (0.22–2.09) |
10 |
1.63 (0.38–3.00) |
12 |
0.20 (0.87–0.31) |
7 |
|
S508T (n = 4) |
0.33 (0.28–0.49) |
8 |
0.16 (0.11–0.20) |
8 |
1.12 (0.56–2.00) |
16 |
0.93 (0.30–3.02) |
7 |
0.22 (0.18–0.33) |
7 |
|
46‐bp insert (n = 1) |
0.40 | 10 | 0.15 | 8 | 1.14 | 16 | 3.17 | 22 | 0.31 | 10 |
|
151‐bp insert (n = 1) |
0.57 | 14 | 0.27 | 14 | 1.59 | 23 | 3.77 | 27 | 0.36 | 12 |
|
232‐bp insert (n = 3) |
0.44 (0.39–0.49) |
11 |
0.16 (0.14–0.19) |
8 |
0.91 (0.79–1.10) |
13 |
4.04 (3.40–4.50) |
29 |
0.24 (0.12–0.55) |
8 |
|
S508T/151‐bp insert (n = 1) |
1.21 | 30 | 0.34 | 17 | 3.10 | 44 | 5.55 | 39 | 0.41 | 14 |
a
Resistance factors (RF) were calculated as the ratio of the EC50 to the mean EC50 values of isolates obtained between 1993 and 1995. Mean EC50 values were calculated on a logarithmic scale and back‐transformed.
b
Number of isolates with the CYP51 variant tested.