Table 2.
Azole sensitivities of S accharomyces cerevisiae YUG37::erg11 transformants
| Transformant | Introduced amino acid substitution(s) | Tebuconazole | Metconazole | Prochloraz | Prothioconazole | Flusilazole | Cycloheximide | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EC50 a (μg/mL) | RFb | EC50 (μg/mL) | RF | EC50 (μg/mL) | RF | EC50 (μg/mL) | RF | EC50 (μg/mL) | RF | EC50 (μg/mL) | RF | ||
| YUG37::erg11_Pb51wt | None | 0.005 | 1 | 0.001 | 1 | 0.003 | 1 | 0.018 | 1 | 0.005 | 1 | 0.046 | 1 |
| YUG37::erg11_Pb51S508T | S508T | 0.036 | 8 | 0.007 | 7 | 0.018 | 6 | 0.067 | 4 | 0.061 | 11 | 0.071 | 2 |
| YUG37::erg11_Pb51G460S/S508T | G460S and S508T | 0.16 | 35 | 0.028 | 26 | 0.034 | 12 | 0.15 | 9 | 0.11 | 21 | 0.056 | 1 |
| Least significant ratioc | 2.1 | 4.5 | 12.5 | 1.1 | 4.0 | 1.7 | |||||||
a
EC50 values are means of results from two independent replicates calculated on a logarithmic scale and back‐transformed.
b
Resistance factors (RF) were calculated as the ratio of EC50 to the EC50 of transformants expressing wild‐type PbCYP51.
c
Back‐transformed values differing by a ratio greater than this are significantly different at P = 0.05.