Skip to main content
. 2014 Feb 8;15(5):466–478. doi: 10.1111/mpp.12109

Figure 5.

figure

A role for the secretory pathway in the subcellular localization of Wheat yellow mosaic virus  P2 and P3. (A) Nicotiana benthamiana leaves expressing P2‐ or P3‐enhanced green fluorescent protein (eGFP) were infiltrated with 10 mg/mL brefeldin A solution, or P2‐ or P3‐eGFP was co‐expressed with Sar1[H74L] in leaves. (B) Bimolecular fluorescence complementation (BiFC) assay to examine the interaction of P2 or P3 with Sar1. Leaves of N. benthamiana plants were infiltrated with mixtures of agrobacteria harbouring the constructs indicated above and on the left side of the images. Fluorescent proteins were observed using confocal laser scanning microscopy (CLSM) at 3 days after inoculation (dai). Bars, 10 μm.