Table 2. Primer sequences, SSR motifs and allele sizes of 99 Fusarium oxysporum f. sp. niveum isolates.
| Primer | Primer sequence (5’–3’) | Ta* | SSR motif | Allele size |
|---|---|---|---|---|
|
MB2 |
F: TGCTGTGTATGGATGGATGG R: CATGGTCGATAGCTTGTCTCAG |
57 |
(GT)11(GA)6 |
234, 237, 238, 240, 242, 246, 248, 250, 252, 254, 260, 264, 271, 275 |
| MB5 |
F: ACTTGGAGGAAATGGGCTTC R: GGATGGCGTTTAATAAATCTGG |
54 | (TG)9 | 252, 254, 256, 267, 274, 344 |
| MB9 |
F: TGGCTGGGATACTGTGTAATTG R: TTAGCTTCAGAGCCCTTTGG |
51 | (CA)9 | 126, 105, 130, 141, 234, 237, 240, 254 |
| MB10 |
F: TATCGAGTCCGGCTTCCAGAAC R: TTGCAATTACCTCCGATACCAC |
48 | (AAC)6 | 206, 208 |
| MB11 |
F: GTGGACGAACACCTGCATC R: AGATCCTCCACCTCCACCTC |
68 | (GGC)7 | 172, 175, 177, 180, 182, 186 |
| MB13 |
F: GGAGGATGAGCTCGATGAAG R: CTAAGCCTGCTACACCCTCG |
68 | (CTTGGAAGTGGTAGCGG)14 | 144, 264, 296, 376, 382, 345, 395, 400, 422, 476, 483, 492, 500 |
| MB14 |
F: CGTCTCTGAACCACCTTCATC R: TTCCTCCGTCCATCCTGAC |
57 | (CCA)5 | 183, 184,186 |
| MB17 |
F: ACTGATTCACCGATCCTTGG R: GCTGGCCTGACTTGTTATCG |
57 | (CA)21 | 299, 301, 303, 308, 312, 317, 319, 320, 321, 331, 334, 337, 339 |
| MB18 |
F: GGTAGGAAATGACGAAGCTGAC R: TGAGCACTCTAGCACTCCAAAC |
57 | (CAACA)6 | 284, 289, 293 |
| Fo9 |
F: GGCAGAAAAGATACTGAACG R: TTGAATTGCCAACTCTTCTT |
55 | (GGA)11 | 204 |
| Fo120 |
F: GAAAGTGGATGGAAGAAAGA R: GATAGGCTGTTGTTGTGGTT |
54.8 | (CAG)7 | 300 |
| Fo310 |
F: CATTGCAGCAGGAATTAGAT R: CTAGGTAGGCATACGAGGGT |
55.6 |
(CA)7 | 318 |
| Fo314 |
F: GAAAAGGAGAGACTGCAAAA R: CTCTTCTTCCTCGTGTTGAC |
54.8 |
(GAA)4 (GAG)9 (GAG)4 |
297 |
| Fo671 |
F: TGTTCCCTGAGTTGGTAGTC R: CTCCCAATCAGATCCTTCT |
55.0 | (CAG)8 | 111 |
| Fo1513 |
F: TGTTCCCTGAGTTGGTAGTC R: CTCCCAATCAGATCCTTCT |
55.0 | (GGA)9 | 125 |
* PCR annealing temperature.