Fig 7. Stress-dependent protein aggregation correlates with dependence on Hsp90 for stability.

(A) Identification of stress granules and P-bodies in C. albicans. Cells were grown overnight in YPD, diluted to an OD₆₀₀ of 0.1, and subcultured for 4 hours before a 10-minute incubation at 46°C. Scale bar is 10 μm. (B) Protein aggregation depends on temperature. Cells were grown overnight in YPD, diluted to an OD₆₀₀ of 0.1, and subcultured for 4 hours at 30°C before a 10-minute incubation at the indicated temperatures. Scale bar is 10 μm. Arrows indicate aggregates. GFP, green fluorescent protein; OD₆₀₀, optical density at wavelength 600 nm; P-body, processing body; RFP, red fluorescent protein; YPD, yeast extract peptone dextrose.