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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: J Am Chem Soc. 2019 Mar 26;141(14):5692–5698. doi: 10.1021/jacs.8b09665

Figure 2.

Figure 2.

(A) The expected secondary structures of the PreQ1-RS constructs in the unbound (w/o Lig) and bound (w Lig) states. (B) PCA plot of the U9, U11, and U14 modified RNA in the absence (w/o Lig) and presence (w Lig) of PreQ1 ligand. All constructs were predicted to be the correct structure in both the unbound and bound states. Condition (A): 10 mM NaH2PO4, 25 mM NaCl, 4 mM MgCl2, 0.5 mM EDTA, pH 7.3 at 25 °C.