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. 2019 Jul 18;8:e45292. doi: 10.7554/eLife.45292

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© 2019, Nimmo et al

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Figure 1. — Left, pAmpPSBA. Right, pAmpChl. Origins of replication (ori for E. coli plasmid, core region for A. carterae minicircle) are shown in red. Protein-coding genes (encoding ampicillin resistance, chloramphenicol resistance or PsbA) are shown in green. The blue shows the original source of of the genetic material (E. coli plasmid and A. carterae minicircle). The red arrow showing the position of the mutation in psbA that confers resistance to atrazine is marked with ‘M’. Primer sites pAmpPSBA 1: MC-pG-F-II, 2: MC-pG-F, 3: MC-pG-R-II, 4: MC-pG-R; pAmpChl: 5: CAT-R, 6: CAT-F, 7: CAT-FSS. CAT-F-Nest and CAT-R-Nest are immediately adjacent to CAT-F and CAT-R, respectively.