Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (Amphidinium carterae) | A. carterae CCMP1314 | Culture Collection of Marine Phytoplankton | CCMP1314 | |
Genetic reagent (vector) | pAmpChl | this paper, synthesized by GeneArt | Full sequence provided in supplemental data. | |
Genetic reagent (vector) | pAmpPSBA | this paper | Full sequence provided in supplemental data. | |
Antibody | Rabbit anti-Chloramphenicol-acetyl-transferase, polyclonal | Antibodies-Online | Antibodies-Online Cat# ABIN285051, RRID:AB_10781219 | 1:500 in f/2 medium with 5% BSA, 1 hr. |
Antibody | Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 405, polyclonal | ThermoFisher | Thermo Fisher Scientific Cat# A-31556, RRID:AB_221605 | 1:1000 in f/2 medium with 5% BSA, 1 hr. |
Commercial assay or kit | DNAdelTM Gold Carrier Particles Optimized for Plasmid Delivery | Seashell Technology | Seashell Technology Cat# S550d |