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. 2019 Jul 10;8:e44752. doi: 10.7554/eLife.44752

Figure 1. Disrupting localization of APC-dependent RNAs, through competition, does not alter their translation.

Figure 1.

(A) Outline of experimental procedure. Sucrose gradients are divided into four fractions based on UV absorbance, an equal amount of spike RNA is added to each, and RNA presence is quantitatively assessed with nanoString analysis. (B, C) Representative absorbance profiles of polysome gradients of control, puromycin treated (B) or Pkp4-cUTR-expressing cells (C). Inset in (B) shows an enlargement of the polysome region. (D) Heat maps showing RNA presence in polysome gradient fractions, based on nanoString analysis, under the indicated conditions. Gene names are shown on the left. Values indicate averages of 3 independent experiments. Statistically significant differences compared to the corresponding control fractions are indicated by asterisks (2-way ANOVA with Dunnett’s multiple comparisons test).

Figure 1—source data 1. File containing values used for generation of the heatmaps and statistics of Figure 1D.
elife-44752-fig1-data1.xlsx (27.7KB, xlsx)
DOI: 10.7554/eLife.44752.003