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. 2019 Jul 10;8:e44752. doi: 10.7554/eLife.44752

Figure 2. Disrupting localization of APC-dependent RNAs, through perturbation of detyrosinated microtubules, does not alter their translation.

Figure 2.

(A) Schematic on the left indicates experimental procedure used for isolation of protrusions. Migration of cells through microporous filters was induced by addition of LPA and protrusion (Ps) and cell body (CB) samples were isolated from control or parthenolide (PTL) treated cells. The indicated RNAs were detected through nanoString analysis to calculate Ps/CB enrichment ratios (n = 3; error bars: standard error). *: p-value<0.04 by two way ANOVA with Bonferroni’s multiple comparisons test against the corresponding control. Parthenolide treatment specifically reduces the enrichment of APC-dependent RNAs at protrusions. (B) Representative absorbance profiles of polysome gradients of control and PTL-treated cells, and heat maps showing RNA presence in polysome gradient fractions, based on nanoString analysis. Gene names are shown on the left. Values indicate averages of 3 independent experiments. No statistically significant differences were detected by 2-way ANOVA with Dunnett’s multiple comparisons test against the corresponding control fractions.

Figure 2—source data 1. File containing values used for generation of the heatmaps and statistics of Figure 2B.
elife-44752-fig2-data1.xlsx (17KB, xlsx)
DOI: 10.7554/eLife.44752.005