Video 1. NIH/3T3 cell expressing scFv-GFP (green), 3x-mCherry-PCP (red) and the control translation reporter.

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DOI: 10.7554/eLife.44752.008

Frames were acquired sequentially and with no time delay, for the duration of the movie (45 s). A merged image of the two channels is shown. Overlapping red and green spots indicate translation sites. Blue line: cell outline. Cyan line: nucleus outline. Scale bar: 5 μm. Single frames of this movie are shown in Figure 3B.