Video 2. NIH/3T3 cell expressing scFv-GFP (green), 3x-mCherry-PCP (red) and the control translation reporter.

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DOI: 10.7554/eLife.44752.013

Frames were acquired sequentially and with no time delay, for the duration of the movie (13 s). A merged image of the two channels is shown. Blue line: cell outline. Scale bar: 5 μm. Single frames of this movie are shown in Figure 4A (upper panels).