. 2019 Jul 10;8:e44752. doi: 10.7554/eLife.44752

Video 4. NIH/3T3 cell expressing scFv-GFP (green), 3x-mCherry-PCP (red) and the localized translation reporter carrying the Rab13 UTR.

DOI: 10.7554/eLife.44752.015

Frames were acquired sequentially and with no time delay, for the duration of the movie (63 s). A merged image of the two channels is shown. Blue line: cell outline. Cyan line: nucleus outline. Scale bar: 5 μm. Single frames of this movie are shown in Figure 4A (bottom panels). RNAs are translated both in the periphery and internal regions.