. 2019 Jul 10;8:e44752. doi: 10.7554/eLife.44752

Video 5. NIH/3T3 cell expressing scFv-GFP (green), 3x-mCherry-PCP and the localized translation reporter carrying the Rab13 UTR.

DOI: 10.7554/eLife.44752.016

Images of the GFP channel were acquired sequentially and with no time delay, for the duration of the movie (13 s). Blue line: cell outline. Scale bar: 3 μm. Single frames of this movie are shown in Figure 4—figure supplement 3A. The edge of the protrusion is towards the top. One of the observed translation sites moves in an apparently directed manner towards the edge of the protrusion.