Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | MDA-MB-231 | ATCC | ATCC Cat# HTB-26, RRID: CVCL_0062 | |
| Cell line (Mus musculus) | NIH/3T3 | ATCC | RRID:CVCL_0594 | |
| Cell line (Homo sapiens) | Primary dermal fibroblasts | provided by Dr. Ramiro Iglesias-Bartolome (NCI, NIH) | ||
| Cell line (Mus musculus) | NIH/3T3-PCFvGdelta1-PP7 (control translation reporter) | This study | NIH/3T3 cells expressing SunTag-based control translation reporter. | |
| Cell line (Mus musculus) | NIH/3T3-PCFvGdelta1-deltaPP7 (control translation reporter) | This study | NIH/3T3 cells expressing SunTag-based control translation reporter with deletion of the PP7 repeats. | |
| Cell line (Mus musculus) | NIH/3T3-PCFvGdelta1-Rab13U (translation reporter/Rab13 UTR) | This study | NIH/3T3 cells expressing SunTag-based translation reporter with the mouse Rab13 UTR. | |
| Cell line (Mus musculus) | NIH/3T3-PCFvGdelta1-Pkp4U (translation reporter/Pkp4 UTR) | This study | NIH/3T3 cells expressing SunTag-based translation reporter with the mouse Pkp4 UTR. | |
| Cell line (Mus musculus) | NIH/3T3-tdMCP-GFP_pIND20-b24bs/Net1 (MS2 reporter/Net1 UTR) | This study | NIH/3T3 cells expressing MS2 reporter with the mouse Net1 UTR. | |
| Cell line (Mus musculus) | NIH/3T3-tdMCP-GFP_pIND20-b24bs/Rab13 (MS2 reporter/Rab13 UTR) | This study | NIH/3T3 cells expressing MS2 reporter with the mouse Rab13 UTR. | |
| Antibody | anti-Rab13 rabbit polyclonal | Novus Biologicals | NBP1-85799 | (1:1,000 WB; 1:200 PLA) |
| Antibody | anti-GAPDH (14C10) rabbit monoclonal | Cell Signaling | 2118 | (1:2,000 WB) |
| Antibody | anti-puromycin (3RH11) mouse monoclonal | Kerafast | EQ0001 | (1:2,000 PLA) |
| Recombinant DNA reagent | pHR-tdPP7-3x-mCherry | Addgene | 74926 | |
| Recombinant DNA reagent | pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 | Addgene | 74928 | |
| Recombinant DNA reagent | pcDNA4TO-24xGCN4_v4-kif18b | Addgene | 74934 | |
| Recombinant DNA reagent | pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE | Addgene | 60906 | |
| Recombinant DNA reagent | pHR-scFv-GCN4-sfGFP-GB1-deltaNLS-dWPRE | This study | Plasmid expressing scFv against GCN4 peptide of the SunTag system, fused to sfGFP, without NLS. Derived from pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE after introduction of stop codon before the NLS sequence. | |
| rRcombinant DNA reagent | pInducer 20 | Addgene | 44012 | |
| Recombinant DNA reagent | Phage-ubc-nls-ha-tdMCP-gfp | Addgene | 40649 | |
| Recombinant DNA reagent | pInducer20-24xGCN4_v4-kif18b-24xPP7-Rab13 UTR | This study | Dox-inducible translation reporter carrying the mouse Rab13 3'UTR | |
| Recombinant DNA reagent | pInducer20-24xGCN4_v4-kif18b-24xPP7-Pkp4 UTR | This study | Dox-inducible translation reporter carrying the mouse Pkp4 3'UTR | |
| Recombinant DNA reagent | pInducer20-beta24bs-Rab13 UTR | This study | Dox-inducible MS2 reporter carrying the mouse Rab13 3'UTR | |
| Recombinant DNA reagent | pInducer20-beta24bs-Net1 UTR | This study | Dox-inducible MS2 reporter carrying the mouse Net1 3'UTR | |
| Sequence-based reagent | All Stars Negative control siRNA | Qiagen | 1027281 | |
| Sequence-based reagent | Rab13 siRNA, si-Rab13 #8 | Qiagen | SI02662702 | target sequence: 5’-ATGGTCTTTCTTGGTATTAAA-3’ |
| Sequence-based reagent | FISH probes against mouse Net1 | Thermo Fisher Scientific | VB1-3034209 | |
| Sequence-based reagent | FISH probes against mouse Cyb5r3 | Thermo Fisher Scientific | VB1-18647 | |
| Sequence-based reagent | FISH probes against mouse Cenpb | Thermo Fisher Scientific | VB1-18648 | |
| Sequence-based reagent | FISH probes against mouse Rab13 | Thermo Fisher Scientific | VB1-14374 | |
| Sequence-based reagent | FISH probes against human Rab13 | Thermo Fisher Scientific | VA1-12225 | |
| Sequence-based reagent | FISH probes against mouse Pkp4 | Thermo Fisher Scientific | VB4-600264 | |
| Sequence-based reagent | FISH probes against human Kif18b | Thermo Fisher Scientific | VA6-3170686 | |
| Sequence-based reagent | FISH probes against mouse Ddr2 | Thermo Fisher Scientific | VB1-14375 | |
| Sequence-based reagent | FISH probes against mouse Arpc3 | Thermo Fisher Scientific | VB1-14507 | |
| Sequence-based reagent | FISH probes against mouse P4hb | Thermo Fisher Scientific | VB6-15898 | |
| Sequence-based reagent | Custom-made codeset | NanoString Technologies | Item # 116000002 | |
| Commercial assay or kit | Duolink In Situ Red kit | Sigma Aldrich | DUO92101 | |
| Commercial assay or kit | ViewRNA ISH Cell Assay kit | Thermo Fisher Scientific | QVC0001 |